Alpha Health Life Scientific
Alpha For The Alpha
Every Single Life Comes From
Stem Cell
What is
Mesenchymal Stem Cell
Mesenchymal stem cells (MSCs) are a kind of multipotent adult stem cells that are distributed in the capillaries of multiple tissues of the human body and have the ability to self-renew, multi-lineage differentiation and secretome. Many researchers consider the transplantation of MSCs to be the most effective tool for cell therapy.
Identify MSCs
Minimal Criteria by ISCT
01
Plastic
Adherent
Be plastic-adherent when maintained in standard culture conditions.
02
03
Multi-lineage Differentiation
CD Markers
Expression
Differentiate into osteoblast, adipocytes, and chondroblasts in vitro.
Positive：CD105, CD90, CD73
Negative：CD45, CD34, CD11b, CD19, HLA-DR.
Clinical Potential
207
Trials
507
Conditions
By Dec 2019, 207 clinical trials associated with MSCs inside of the U.S. were enrolled into clinicaltrials.gov after submitting to FDA, and covered 507 conditions totally.
Disclaimer: All clinical information provided herein is based solely on data from ongoing clinical trials listed on clinicaltrials.gov. This information is intended for educational purposes only and should not be used for therapy, diagnosis, or any other medical application.
Reference：clinicaltrials.gov
Mechanisms
247
Proteins
201
Genes
58
Processes
30
Pathways
Immune Privilege
The absence of major histocompatibility complex (MHC)/human leukocyte antigen(HLA) class II and associated costimulatory molecules and low levels of MHC/HLA class I.
Migration
Pathological tissue releases a variety of inflammatory factors, prompting MSCs to accumulate at sites through microvessels, thereby releasing cytokines to repairing.
Paracrine
Secret a wide array of bioactive factors such as growth factors, cytokines, and chemokines to activate endogenous stem cells and regulate immune cells.
Exosomes
Extracellular vesicles that contain proteins, lipids and RNAs reduce the secretion of pro-inflammatory cytokines and increase the production of TGF-β.
Immuno-
modulatory
Mitochondrial
Transfer
Through cell-to-cell contact and paracrine, MSCs modulate the proliferation, differentiation, and mechanisms of immune cells to regulate the immune system and suppress inflammation.
The capability of MSCs to transfer their own mitochondria into mtDNA depleted cells was shown with a high expression of RHOT1.
Proliferation
Potential
Highly proliferative potential and telomerase activity. The proliferation rate is no significantly reduced and the karyotype is stable even for 30 passages.
Angiogenesis
Promote angiogenesis through paracrine angiogenic factors (VEGF, IL-6, etc.) and promote endothelial cell proliferation and migration.
Multilineage
Differentiation
MSCs showed high differentiation capacity, not only osteoblasts, adipocytes, chondrocytes, but also cardiomyocytes, smooth muscle cells, etc.
Paracrine
Interaction with Endogenous cells
MSCs produce a variety of bioactive factors such as growth factors, cytokines, and chemokines that interact with endogenous cells to stimulate innate tissue repair and modulate inflammation and immune responses.
Examples: MSCs produce IL-6 to acquire tolerogenic phenotypes, PGE2 to suppress NK cells cytotoxicity and CD4+ and CD8+ T-cell proliferation, and IDO to inhibit the differentiation of circulating T follicular helper cells.
Reference: Pittenger MF, et al. Mesenchymal stem cell perspective: cell biology to clinical progress. NPJ Regen Med.2019 Dec 2;4:22.
Why From
Umbilical Cord
Tissue is NOT Stem Cell
Umbilical Cord Tissue
Tissue Product
PHS Act 361 & 21 CFR Part 1271
Minimal Manipulation
Homologous Use Only
45 minutes Online Registration
on Form FDA 3486
MSCs Purity<1%
Cell Viability<40%
Low Biological Potency
Frozen Tissue Solution
Cubic Centimeter/ ml
≠
Mesenchymal Stem Cells
Investigational Cell Drug
PHS Act 351 & 21 CFR Part 312
Culture & Expansion
Clinical Use in Human
Pre-clinical Experiments: 5-10 years
Technology Transfer+IND Submission
FDA Review: 30 days+Several Months
Clinical Trials: 5 years
MSCs Purity>95%
Cell Viability>90%
High Biological Potency
Fresh Cell Suspension
Cell Count
Warnings
From FDA
On behalf of patients, the FDA advised patients who need stem cell treatment only consider either:
-
FDA-approved, or;
-
Being studied in clinical trials under an Investigational New Drug Application (IND).
Before getting treatment, ask your provider to give you the information of FDA-issued Investigational New Drug Application, and sign a consent form that explains the experimental procedure.
